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You are here:Home » Antibodies » Abs to Enzymes, Decarboxylase » Anti -Glutamate Decarboxylase 67 (GAD67)

Anti -Glutamate Decarboxylase 67 (GAD67)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Glutamic acid decarboxylase (GAD) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid
(GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65kD and 67kD, 64% amino acid identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. GAD67 is cytoplasmic (594aa), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation or disease progression.
Catalog #G4000-20
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000-1:10,000; Using ECL on rat and mouse brain lysate.
Immunohistochemistry (Paraffin): 1:500; No epitope retrieval necessary.
Optimal dilutions to be determined by the researcher.
Positive ControlSKNSH cell lysate (human neuroblastoma), mouse cerebral cortex
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H404
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.02M PBS, pH 7.6, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant rat GAD67 protein
SpecificityRecognizes rat Glutamate Decarboxylase 67 at ~67kD. No detectable crossreactivity with GAD65 by Western Blot on rat brain lysate. Species Crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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