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You are here:Home » Antibodies » Abs to Enzymes, Dehydrogenase » Anti -Glutamate Dehydrogenase (GDH, GLDH, GLUD 1, GLUD, GLUD1, GLUD1, Glutamate dehydrogenase 1, Glutamate dehydrogenase 1 mitochondrial, MGC132003)

Anti -Glutamate Dehydrogenase (GDH, GLDH, GLUD 1, GLUD, GLUD1, GLUD1, Glutamate
dehydrogenase 1, Glutamate dehydrogenase 1 mitochondrial, MGC132003)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B
Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase GDH1, that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix.
Catalog #G4000-51
ApplicationsSuitable for Immunoblotting (western or dot blot), ELISA, and conjugation. Other applications have not been tested.
Recommended DilutionELISA: 1:5000 -1:20,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~10mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol.
PurityPuri ed by delipidation, fractionation and ion exchange chromatography.
ImmunogenBovine liver glutamate dehydrogenase.
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum as well as purified and partially purified bovine liver glutamate dehydrogenase.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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