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You are here:Home » Antibodies » Abs to Enzymes, Dehydrogenase » Anti -Glutamate Dehydrogenase (GDH, GLDH, GLUD 1, GLUD, GLUD1, GLUD1, Glutamate dehydrogenase 1, Glutamate dehydrogenase 1 mitochondrial, MGC132003) (HRP)

Anti -Glutamate Dehydrogenase (GDH, GLDH, GLUD 1, GLUD, GLUD1, GLUD1, Glutamate
dehydrogenase 1, Glutamate dehydrogenase 1 mitochondrial, MGC132003) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH
Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase GDH1, that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix.
Catalog #G4000-65
ApplicationsSuitavle for use in ELISA, Western Blot, Dot Blot, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:1000-1:4000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~10mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 0.01% gentamicin sulfate, 10mg/ml BSA (IgG and Protease free), 40% glycerol. Labeled with horseradish peroxidase (HRP).
PurityPuri ed by delipidation, fractionation and ion exchange chromatography.
ImmunogenBovine liver glutamate dehydrogenase.
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against bovine liver glutamate dehydrogenase, anti-HRP and anti-rabbit serum. Crossreactivity from other tissues and species may occur but have not been determined.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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