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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -Glutamate Transporter, Aspartate EAAT1 (GLAST)

Anti -Glutamate Transporter, Aspartate EAAT1 (GLAST)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4 and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Glutamate transporters (525-573aa) display about 55% homology. They are predicted to contain up to 6-10 transmembrane domains. Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord. EAAC1 is specific for certain neurons and Purkinje cells. It is specifically enriched in cortex, hippocampus and caudate-putamen. It is confined to presynaptic and postsynaptic elements. GLAST has been observed in both neuron and astroglia. It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum. EAAT4 has properties of ligand gated Cl-channel. It is localized mainly in cerebellar Purkinje cells in rat and human CNS. EAAT5 has only been cloned from human. It is primarily expressed in retina. GLAST (sodium dependent Glutamate/Aspartate Transporter 1, or Excitatory Amino Acid Transporter 1, EAAT1) is a glutamate transporter. It is a transmembrane protein with ~543aa and a molecular weight of 67kD.
Catalog #G4003-90B
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5,000
ELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 5-10ug/1ul serum.
Optimal dilutions to be determined by the researcher.
Control PeptideG4003-90A: Glutamate Transporter, Aspartate EAAT1, Rat (GLAST)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
PuritySerum
ImmunogenSynthetic peptide (15aa) near the C-terminus of rat GLAST (KLH coupled).
SpecificityRecognizes rat GLAST. No significant sequence homology to other glutamate transporters. Species Sequence Homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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