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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -Glutamate Transporter, Neuronal (EAAC1, EAAT3)

Anti -Glutamate Transporter, Neuronal (EAAC1, EAAT3)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Serum IH
The antibody has been tested on central nervous system tissue. The staining pattern for the cell bodies obtained corresponds to the pattern described using in situ hybridization with probes to rEAAC1 mRNA. The immunoreactivity corresponds to what has been described for glutamatergic neuronal systems. Preadsorption of the antiserum with the immunogen peptide completely abolishes the immunostaining
Catalog #G4005-40
ApplicationsSuitable for use in Immunohistochemistry. Not recommended for use in Western blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:4000-1:8000 using a cyanine-conjugated secondary antibody. Enzymatic methods of detections can be used, however antibody dilution must be increased to 1:10,000-1:30,000. Lightly fixed, 4% PFA material and the use of triton X-100 in the block only is recommended.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
ConcentrationNot Determined
FormSupplied as a liquid. No preservatives added.
PuritySerum
ImmunogenSynthetic peptide corresponding to the carboxy-terminus of the cloned rat EAAC1.
SpecificityRecognizes rat Neuronal Glutamate Transporter (EAAC1; also called EAAT3). Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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