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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP

L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. To maintain glutamate levels below excitoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identi ed: the sodium-dependent GLAST-1, GLT-1, EAAC-1 and the chloride-dependent EAAT-4. EAAC-1 is expressed in the CNS and also found in kidney and small intestine. Structural features of glutamate transporters (2) are believed to include eight membrane-spanning alpha-helices and a loop-pore structure which is unique among secondary transporters but may resemble loop-pores found in ion channels. A second distinctive structural feature is the presence of a highly amphipathic membrane-spanning helix that provides a hydrophilic path through the membrane. Mice with homozygous deletions of the EAAC-1 transporter gene (3) develop dicarboxylic aminoaciduria and display reduced spontaneous locomotor activity although no neurodegeneration was observed over a period of 12 months.
Catalog #	G4005-50A
Applications	Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	ELISA: 0.1-1ug/ml
	Western Blot: 1-5ug/ml
	Immunoprecipitation: 1-5ug/IP reaction
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	3G157
Host	Mouse
Source	Rat
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide corresponding to amino acids 161-177 of the rat glutamate transporter EAAC1 sequence.
Specificity	Recognizes the ~70kD glutamate transporter EAAC1. Species Crossreactivity: rat and human glutamate transporter EAAC1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.