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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E IH IC

L-Glutamate is a non-essential amino acid naturally occurring in the L-form. It is the most common excitatory neurotransmitter in the Central Nervous System.
Catalog #	G7115-01
Applications
	Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Specificity	Using a conjugate L-Glutamate-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
	Compound Crossreactivity Ratio L-Glutamate-G-BSA 1D-Glutamate-G-BSA 1/>50,000L-Aspartate-G-BSA 1/>50,000D-Aspartate-G-BSA 1/>50,000GABA-G-BSA 1/>50,000
(a)	Glutamate-G-BSA concentration/unconjugated or conjugated amino acid concentration at half displacement.
	G = Glutaraldehyde, BSA = Bovine Serum Albumin
Recommended Dilutions	Immunocytochemistry: 1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2. No preservative added.
Purity	Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Immunogen	Synthetic L-Glutamate conjugated to protein carrier
Specificity	Recognizes protein-linked L-Glutamate. See additional crossreactivity data.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.