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You are here:Home » Antibodies » Abs to Amino Acids » Anti -L-Glutamate, Conjugated

Anti -L-Glutamate, Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E IH IC
L-Glutamate is a non-essential amino acid naturally occurring in the L-form. It is the most common excitatory neurotransmitter in the Central Nervous System.
Catalog #G7115-01
Applications
Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
SpecificityUsing a conjugate L-Glutamate-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
Compound Crossreactivity Ratio L-Glutamate-G-BSA 1 D-Glutamate-G-BSA 1/>50,000 L-Aspartate-G-BSA 1/>50,000 D-Aspartate-G-BSA 1/>50,000 GABA-G-BSA 1/>50,000
(a) Glutamate-G-BSA concentration/unconjugated or conjugated amino acid concentration at half displacement.
G = Glutaraldehyde, BSA = Bovine Serum Albumin
Recommended DilutionsImmunocytochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityAntiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
ImmunogenSynthetic L-Glutamate conjugated to protein carrier
SpecificityRecognizes protein-linked L-Glutamate. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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