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You are here:Home » Antibodies » Abs to Enzymes, Decarboxylase » Anti -Glutamic Acid Decarboxylase (GAD)

Anti -Glutamic Acid Decarboxylase (GAD)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH
The enzyme glutamic acid decarboxylase (GAD) catalyzes the synthesis of g-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. GAD is the rate-limiting enzyme in the biosynthesis of GABA from L-glutamic acid, and it is used extensively as a marker for GABAergic neurons and synaptic terminals. GAD has been observed in pancreatic islet cells and identified as an autoantigen associated with the development of insulin-dependent diabetes mellitus.
Catalog #G7116-05
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1ug/ml
Optimal dilutions to be determined by the researcher.
Immunohistochemistry Protocol1. Perfuse rats with 100mM phosphate buffer, pH 7.4, containing 1% paraformaldehyde, 0.34% L-lysine and 0.05% m-periodate (1% PLP).
2. Postfix brains in 1% PLP for 1-2 hours.
3. Transfer brains to 100mM phosphate buffer containing 30% sucrose and gently agitate on a shaker platform at 4°C for 48-60 hours.
4. Using a sliding microtome, cut 30um sections of frozen cerebellum. As the sections are cut, collect them in a vial of cold 100mM phosphate buffer.
5. Incubate sections in PBS containing 1.5% normal serum and 0.2% Triton X-100 for 30 minutes.
6. On a shaker platform, incubate sections with G7116-05
(diluted 1ug/ml in PBS containing 1.5% normal serum and 0.2% Triton X-100) for 12-36 hours at 4°C.
7. On a shaker platform, rinse sections eight times, 10-15 minutes per rinse, in PBS.
8. Detect with standard secondary antibody detection system
(PAP, ABC, etc.).
9. Mount sections, dehydrate, and apply coverslips.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q61
HostMouse
SourceRat
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 100ul sterile 40-50% glycerol, ddH2O.
PurityPurified immunoglobulin
ImmunogenPurified rat brain glutamic acid decarboxylase (GAD).
SpecificityRecognizes the lower MW isoform (65kDa) of the two GAD isoforms identified in brain. This monclonal antibody can be used for immunohistochemical localization in brain or pancreas. Has been used to label purified GAD on Western blots.
Species CrossreactivityRat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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