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You are here:Home » Antibodies » Abs to Enzymes, Synthetase » Anti -Glutamine Synthetase

Anti -Glutamine Synthetase

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 45kD subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.
Catalog #G7121
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested
Recommended DilutionsWestern Blot: 1:500. Detected Glutamine Synthetase on 10ug of human brain lysates. Single band in sheep and rat brain
Immunohistochemistry: Rat brain tissue
Optimal dilutions to be determined by the researcher.
Positive ControlRat brain tissue, rat brain cytosolic fraction, human brain lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q56
HostMouse
SourceSheep
Concentration~1mg/ml
FormSupplied as a liquid in PBS pH 7.5, BSA, <0.09% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenGlutamine synthetase purified from sheep brain (Accession# NM_05178.3).
SpecificityRecognizes sheep Glutamine Synthase at ~45kD.
Species CrossreactivityHuman, mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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