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You are here:Home » Molecular Biology » MB-BioSep Agarose » Glutathione (Sepharose)

Glutathione (Sepharose)


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The Glutathione-S-Transferase (GST) gene fusion system has been widely used for the over expression of foreign genes in E. coli. The expressed fusion protein with a GST tail can be easily purified by affinity chromatography on Glutathione-Sepharose beads from the bacterial lysate. Glutathione-Sepharose is designed for the purpose of purification of such GST fusion proteins or any other kinds of glutathione binding proteins. This formulation exhibits excellent binding capacity, high flow rate, no significant loss of the glutathione ligand and a pH stability range of 2-10.
Catalog #G8123-11
ApplicationsSuitable for use for purification of GST-fusion proteins or other glutathione- binding proteins.
Bead Structure4% cross-linked spherical agarose
Mean particle size90um (45-165um)
pH stability2-10
Chemical stability1M NaOH (1wk, 20°C)
0.01M NaOH , pH 12
0.01M HCl, pH 2
4M sodium chloride
8M urea
6M guanidine hydrochloride
Storage buffer0.01% Thimersol / ddH2O
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
FormSupplied as a 50% slurry in 0.01% Thimerosal / H2O; > 3mg (10umol) glutathione per ml Sepharose beads.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesGlutathione Agarose, Glutathione-Agarose, Glutathione Beads, Glutathione-Sepharose

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