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Specifications

The Glutathione-S-Transferase (GST) gene fusion system has been widely used for the over expression of foreign genes in E. coli. The expressed fusion protein with a GST tail can be easily purified by affinity chromatography on Glutathione-Sepharose beads from the bacterial lysate. Glutathione-Sepharose is designed for the purpose of purification of such GST fusion proteins or any other kinds of glutathione binding proteins. This formulation exhibits excellent binding capacity, high flow rate, no significant loss of the glutathione ligand and a pH stability range of 2-10.
Catalog #	G8123-11
Applications	Suitable for use for purification of GST-fusion proteins or other glutathione- binding proteins.
Bead Structure	4% cross-linked spherical agarose
Mean particle size	90um (45-165um)
Ligand	Glutathione
pH stability	2-10
Chemical stability	1M NaOH (1wk, 20°C)
	0.01M NaOH , pH 12
	0.01M HCl, pH 2
	4M sodium chloride
	8M urea
	6M guanidine hydrochloride
Storage buffer	0.01% Thimersol / ddH2O
Storage and Stability	May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Form	Supplied as a 50% slurry in 0.01% Thimerosal / H2O; > 3mg (10umol) glutathione per ml Sepharose beads.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	Glutathione Agarose, Glutathione-Agarose, Glutathione Beads, Glutathione-Sepharose