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You are here:Home » Antibodies » Abs to Biochemicals » Anti -Glutathione

Anti -Glutathione

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E IH
Catalog #G8123-15
ApplicationsSuitable for use in ELISA, Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: Prepare enough G8125-15 for the day’s use by diluting 100X with 1% GSPBT. Using silver-intensified immunogold or fluorescence. Samples should be fixed with 0.2%–2.5% glutaraldehyde for optimum detection. This antibody has also been used and found to work with a zero-low glutaraldehyde/high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer/3% sucrose fixative). The minimum glutaraldehyde concentration is 0.02%. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: We do not recommend adding glycerol as a cryopreservative.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as a liquid in PBS with sterile goat serum containing 0.05% thimerosal as a preservative.
PurityPurified immunoglobulin
ImmunogenGlutathione-glutaraldehyde
SpecificityRecognizes Glutathione. The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue crossreactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Does not discriminate between free GSH and GSSG.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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