Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E IH

Catalog #	G8123-15
Applications	Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: Prepare enough G8125-15 for the day’s use by diluting 100X with 1% GSPBT. Using silver-intensified immunogold or fluorescence. Samples should be fixed with 0.2%–2.5% glutaraldehyde for optimum detection. This antibody has also been used and found to work with a zero-low glutaraldehyde/high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer/3% sucrose fixative). The minimum glutaraldehyde concentration is 0.02%. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
	Note: We do not recommend adding glycerol as a cryopreservative.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	Not determined
Form	Supplied as a liquid in PBS with sterile goat serum containing 0.05% thimerosal as a preservative.
Purity	Purified immunoglobulin
Immunogen	Glutathione-glutaraldehyde
Specificity	Recognizes Glutathione. The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue crossreactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Does not discriminate between free GSH and GSSG.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.