Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Enzymes, Peroxidase » Anti -Glutathione Peroxidase 3 (GPX3, GSHPx-3, GPx-3, GSHPx-P, GPx-P, GPXP)

Anti -Glutathione Peroxidase 3 (GPX3, GSHPx-3, GPx-3, GSHPx-P, GPx-P, GPXP)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IH IC
Glutathione Peroxidase 3 (GPX3), also known as Plasma Glutathione Peroxidase, is a 226aa member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100kD homotetramer consisting of four identical 23kD subunits, each containing a selenocysteine residue at the active site. GPX3 exhibits highest levels of mRNA expression in liver (human only), kidney, heart and lung tissues. Mouse GPX3 shares 89% and 99% amino acid identity with human and rat GPX3, respectively.
Catalog #G8127-13
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Immunohistochemistry: 2-15ug/ml
Immunocytochemistry: 2-15ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-28K: IgG, H&L (HRP) Pab Rb xGt
I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab Rb xGt
I1904-28V: IgG, (HRP) Pab Rb xGt
I1904-29A: IgG, (HRP) Pab Rb xGt
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 100ul sterile PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa74-226 of mouse GPX3 expressed in E. coli.
SpecificityRecognizes endogenous mouse Glutathione Peroxidase 3 (GPX3). Species Crossreactivity: Human, rat. Does not crossreact with recombinant human GPX1 or GPX2 in Western Blots.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links