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You are here:Home » Antibodies » Abs to GST » Anti -Glutathione-S-Transferase [Schistosoma japonicum] (AP)

Anti -Glutathione-S-Transferase [Schistosoma japonicum] (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
GST (Glutathione S-Transferase) is a 26kD protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
Catalog #G8134
ApplicationsSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:1000-1:5000
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:500-1:2000
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of activity. Alkaline phosphatase conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~1mg/ml
FormSupplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
PurityPurified from by immunoaffinity chromatography.
ImmunogenGlutathione-S-Transferase [Schistosoma japonicum]
SpecificityRecognizes Glutathione-S-Transferase. Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-alkaline phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-STransferase from other sources may occur but has not been specifically determined.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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