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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Glutathione S-Transferase (GST)

Anti -Glutathione S-Transferase (GST)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP IC IF
Epitope tags are useful for thge labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteinís biochemical properties. Glutathione S-transferase (GST) is a widely used protein fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
Catalog #G8135-01
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:200
Immunocytochemistry (for IF): 1:800
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3A18
HostMouse
ConcentrationNot determined.
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Also available without BSA & Azide. See G8135-01G.
PurityAscites
ImmunogenGST fusion protein.
SpecificityRecognizes overexpressed GST fusion proteins. Recognizes only glutathione S-transferase. Species Crossreactivity: It is expected that antibody will react with proteins fused to GST from all species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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