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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Glutathione S-Transferase (GST)

Anti -Glutathione S-Transferase (GST)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B
Glutathione S-transferase is a 26kD protein from Schistosoma japonicum. GST is utilized as a fusion protein in protein expression from a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Catalog #G8135-01A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:50-1:100
Optimal working dilutions to be determined by researcher.
HybridomaNSO myeloma cells with spleen cells from Balb/c mice.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2162
HostMouse
ConcentrationAs reported
FormSupplied as a liquid in 0.2M Tris HCl pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
PurityTissue Culture Supernatant.
ImmunogenRecombinant glutathione-S-transferase (GST), from Schistosoma japonicum.
SpecificityRecognizes GST.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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