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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Glutathione S Transferase (GST) (Biotin)

Anti -Glutathione S Transferase (GST) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Glutathione S-transferase is a 26kD protein from Schistosoma japonicum. GST is utilized as a fusion protein in protein expression from a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Catalog #G8135-01B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:100-1:500
Optimal working dilutions to be determined by researcher.
HybridomaSpleen cells from immunized BALB/c mice were fused with cells of the mouse NSO myeloma cell line.
Recommended Secondary AntibodiesS7973-92F2: Streptavidin (PE)
S7973-96B: Streptavidin (FITC)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2162
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with biotin.
PurityPurified by Protein A affinity chromotography.
ImmunogenRecombinant glutathione-S-transferase (GST) from Schistosoma japonicum.
SpecificityRecognizes GST.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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