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Specifications

Clone Host Grade Applications Monoclonal Rabbit Purified B IP

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
Catalog #	G8135-01C
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	11C1036
Host	Rabbit
Concentration	Not determined
Form	Supplied as a liquid in 136mM sodium chloride, 2.6mM KCl, 12mM sodium phosphate, pH7.4 dibasic, 2 mg/ml BSA, 50% glycerol.
Purity	Purified
Immunogen	Immunizing animals with a GST fusion protein.
Specificity	Recognizes transfected GST fusion proteins. Species Crossreactivity: All.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.