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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Glutathione S Transferase (GST)

Anti -Glutathione S Transferase (GST)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IP
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteinís biochemical properties. Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
Catalog #G8135-01C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C1036
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in 136mM sodium chloride, 2.6mM KCl, 12mM sodium phosphate, pH7.4 dibasic, 2 mg/ml BSA, 50% glycerol.
PurityPurified
ImmunogenImmunizing animals with a GST fusion protein.
SpecificityRecognizes transfected GST fusion proteins. Species Crossreactivity: All.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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