Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IC

Glutathione S-transferase is a 26kD protein from Schistosoma japonicum. GST is utilized as a fusion protein in protein expression from a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immunopurification of GST-fusion proteins.
Catalog #	G8135-02A
Applications	Suitable for use in ELISA, Western Blot and Immunocytochemistry . Other applications not tested.
Recommended Dilution	ELISA:
	Coating: 1:50-1:250
	Primary: 1:500-1:15,000
	Western Blot:
	Colorimetric detection: 1:500-1:5000
	Chemiluminescent detection: 1:500-1:15,000
	Immunocytochemistry: 1:50-1:200
	Optimum dilutions to be determined by researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Host	Rabbit
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Specificity	Recognizes Glutathione S Transferase (GST).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.