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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Catalog #	G8135-05
Applications	Suitable for Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions	Western Blot: 0.5-2ug/ml detects 100ng of a GST fusion protein.
	Immunoprecipitation: 4ug immunoprecipitates GST fusion protein.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	0.T.177
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH.
Specificity	Recognizes GST-fusion proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.