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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Glutathione S Transferase (GST)

Anti -Glutathione S Transferase (GST)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #G8135-08
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 0.5-2ug/ml of this lot detected 100ng of MAP Kinase 2/Erk2, active. 0.5-2ug/ml of a previous lot detected 500ng of GST. Representative blot from a previous lot. Cell lysate containing GST fusion protein was resolved by electrophoresis, transferred to nitro-cellulose and probed with anti-GST (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemilumine-scence detection system. Arrow indicates the GST fusion protein.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride with 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenGST (Glutathione-S-transferase) derived from the pGEX expression vector and linked to KLH.
SpecificityRecognizes GST-fusion proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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