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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Glutathione S Transferase (GST)

Anti -Glutathione S Transferase (GST)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B
Glutathione S-transferase is a 26kD protein from Schistosoma japonicum. GST is utilized as a fusion protein in protein expression from a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Catalog #G8135-09A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml detected recombinant GST protein and GST-Abltide fusion protein. Recombinant GST protein (20ng), and a GST fusion protein (50ng,) were resolved by electrophoresis, transferred to nitrocellulose and probed with G8135-09A (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No4H327
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by ammonium sulfate precipitation.
ImmunogenRecombinant GST (glutathione S-transferase) from Shistosoma japonicum expressed in E. coli.
SpecificityRecognizes GST fusion proteins. Recognizes bacterial GST. Not expected to crossreact with GST from other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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