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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Glutathione S-Transferase (GST)

Anti -Glutathione S-Transferase (GST)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
The approach of epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a GST tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the GST-tagged product.
Catalog #G8135-17A
ApplicationsAffinity Purification (For conjugation, order Ab without BSA)
Western Blot: (Ab 0.5-1ug/ml for 2hrs at RT)
Cellular Localization Depends upon the localization of the parent protein tagged with GST.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5G122
HostMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified recombinant GST protein.
SpecificityReacts with free GST or GST fusion proteins in various immunochemical assays. Species Crossreactivity: Proteins from all species which are tagged with GST.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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