| Catalog # | G8135-32 |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.5-2ug/ml. Detects GST M1(mu) in HeLa cell lysate. Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8135-32 |
| (0.5ug/ml). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP* and a chemiluminescence detection system. |
| Optimal dilution determined by the researcher. |
| *Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide, aa121-134, CEKLKPKYLEELPEK corresponding to mouse GST M1. |
| Specificity | Recognizes mouse GST M1, Mr ~26kD. Recognizes additional non-specific proteins, Mr 66kD and 55kD, following prolonged exposure. Species Crossreactivity: human and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
