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You are here:Home » Antibodies » Abs to Enzymes, Dehydrogenase » Anti -Glyceraldehyde 3 Phosphate Dehydrogenase (GAPD, GAPDH) (Biotin)

Anti -Glyceraldehyde 3 Phosphate Dehydrogenase (GAPD, GAPDH) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. Though expressed differently in different tissues , GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often measured as a control in experiments quantifying specific changes in expression of other targets. Recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntington's and Alzheimer's disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Catalog #G8140-01E
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenGlyceraldehyde 3 Phosphate Dehydrogenase.
SpecificityRecognizes GAPDH. Species Crossreactivity: human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell or tissue lysate
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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