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You are here:Home » Antibodies » Abs to Enzymes, Dehydrogenase » Anti -GAPDH, Loading Control (Glyceraldehyde 3-Phosphate Dehydrogenase)

Anti -GAPDH, Loading Control (Glyceraldehyde 3-Phosphate Dehydrogenase)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, G3PDH or GPDH) is one of the key enzymes involved in glycolysis; it catalyzes the reversible oxidative phosphorylation of glyceraldehydes-3-phosphate. The GAPDH gene is constitutively and stably expressed at high levels in almost all tissues and cells, and as such is considered to be a "housekeeping" gene. Housekeeping proteins like GAPDH are useful as loading controls for western blots and protein normalization.
Catalog #G8140-15H
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: >1:16,000
Western Blot: 0.01-0.1ug/ml
Immunohistochemistry (paraffin): 0.3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlBrain, heart, kidney, liver, lung, stomach, spleen, ovary, testis, and 293. Most cell lines and tissues
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to HQVVSSDFNSDT, from the C-terminus of the protein sequence according to NP_002037.
SpecificityRecognizes GAPDH. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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