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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -Glycine Transporter 2, Neuronal

Anti -Glycine Transporter 2, Neuronal

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Serum IH
Glycine Transporter 2 (GLYT2) is a membrane protein which recaptures glycine, a major inhibitory transmitter in the spinal cord and brainstem. GlyT2 is a specific marker of glycinergic neurons and a member of the Na+ and Cl--coupled transporter family SLC6. Glycine uptake mediated by GlyT2 is electrogenic, coupled to three Na+ and one Cl-. In humans, GlyT2 is encoded by the SLC6A5 gene. Inactivation of GlyT2 in knockout mice is lethal during the second post-natal week as the absence of GlyT2 disrupts inhibitory transmission by reducing glycine release. Mutations in SLC6A5 gene are responsible for a presynaptic form of hyperekplexia, a genetic disease causing increased startle reflex. GlyT2 main physiological role is to recapture glycine released in the synaptic cleft and to maintain high glycine concentration in the presynaptic neuron.
Catalog #G8168-20
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:50001:10,000 using a Cy3 conjugated secondary antibody. In some neuronal systems in the brain the antibody has been used at a dilution of 1:20,000. The tissue was fixed in a paraformaldehyde/picric acid-mixture (4% paraformaldehyde and 0.4% picric acid in 0.16M sodium phosphate buffer, pH 6.9).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGuinea pig
SourceRat
ConcentrationNot Determined
FormSupplied as a liquid. No preservatives
PuritySerum
ImmunogenSynthetic peptide corresponding to aa780-799, GTSSLGLKLPVKDLELGTQC from the carboxy-terminus as predicted from the cloned rat GLYT2.
SpecificityRecognizes rat Glycine Transporter 2, Neuronal (GLYT2). The antiserum has been tested on tissue sections from the central nervous system. The staining pattern obtained corresponds to the pattern described using in situ hybridization with probes to GLYT2 mRNA and to what has been described for glycinergic neurons. Preabsorption of the antiserum with the immunogen peptide completely abolishes the immunostaining.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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