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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Serum IH

Catalog #	G8168-20
Immunohistochemistry	1:5,000–1:10,000 using a Cy3 conjugated secondary antibody. In some neuronal systems in the brain the antibody has been used at a dilution of 1:20,000. The tissue was fixed in a paraformaldehyde/picric acid-mixture (4% paraformaldehyde and 0.4% picric acid in 0.16 M sodium phosphate buffer, pH 6.9).
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Source	Rat
Concentration	Not determined
Form	Liquid, no preservatives.
Purity	Serum
Immunogen	Synthetic peptide (GTSSLGLKLPVKDLELGTQC) corresponding to amino acids 780–799 from the carboxy-terminus as predicted from the cloned rat GLYT2.
Specificity	Glycine Transporter 2, Neuronal (GLYT2). The antiserum has been tested on tissue sections from the central nervous system. The staining pattern obtained corresponds to the pattern described using in situ hybridization with probes to GLYT2 mRNA and to what has been described for glycinergic neurons. Preabsorption of the antiserum with the immunogen peptide completely abolishes the immunostaining. Species Reactivity: Rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.