| Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. The enzyme is the mammalian homolog of the zeste-white3 (shaggy) homeotic gene of Drosophila melanogaster and has been implicated in the regulation of the c-Jun/AP-1 transcription factor. Genetic and biochemical evidence now indicates that GSK-3 plays a role in intracellular signaling pathways. For example, during insulin and growth factor signaling the GSK-3 beta isoform becomes phosphorylated on its N-terminal serine-9 residue, presumably by either the action of MAPKAP Kinase-1 (p90 S6 kinase, Rsk-2) or p70 S6 kinase. Such phosphorylation of GSK-3 blocks its ability to phosphorylate c-Jun, protein phosphatase inhibitor 2 or the GSK-3 substrate peptide. GSK-3 is constitutively phosphorylated at this tyrosine residue in resting cells. This immunogen is identical in 15 of 17 residues in human and rat GSK-3a (Y279) and GSK-3b (Y216). The phosphotyrosine residue can be removed from GSK-3 by treatment with PTP1B. |
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| Catalog # | G8170-47 |
| Applications | Suitable for use in Western Blot. Not suitable for use in Immunoprecipitation. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 0.5-2ug/ml detects phospho-GSK-3 in RIPA lysates from human Jurkat cells. Previous lots detected phospho-GSK-3 in mouse 3T3/A31, human A431 and rat L6 cell lysates. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8170-47. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system |
| Optimal dilutions to be determined by the researcher. |
| Included Positive Antigen Control | J9000: Jurkat lysate. Use 20ug per lane for minigels. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 0.T.57 |
| Host | Mouse |
| Source | Drosophila |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide (aa203-219, C-KQLLHGEPNVS[ pY]ICSRY) corresponding to the catalytic domain (between subdomain VII & VIII) of Drosophila GSK-3/shaggy enzyme coupled to ovalbumin. |
| Specificity | Recognizes Drosophila Glycogen Synthase Kinase 3, phosphorylated (Y279/Y216) (phosphoGSK-3) at 46kD (alpha) and 51kD (beta). Species crossreactivity: mouse, rat, human, S. pombe and Dictyostelium. Expected to crossreact with nearly all species because of the extremely high conservation of this region among the known enzymes of many different species. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
