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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH FC

Glycophorin A is a sialoglycoprotein, expressed on human red blood cell membranes and erythroid precursors, including proerythroblasts, and reticulocytes.
Catalog #	G8177-10X
Applications	Suitable for use in Flow Cytometry, Fluorescent Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Flow Cytometry: 2ug antibody/5x10e6-5x10e7 red blood cells/test (RBCs/test).
	Immunohistochemistry (fixed, paraffin-embedded): 1-2ug/ml
	Optimal dilutions to be determined by the researcher.
Hybridoma	Hybridization of NS/1-Ag4 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	1.B.777
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS. No preservatives added. No stabilizing proteins added. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol, 0.1% sodium azide for long term storage.
Purity	Puri ed
Immunogen	Human red blood cells.
Specificity	Recognizes Glycophorin A.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.