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You are here:Home » Antibodies » Abs to CD Markers » Anti -Glycophorin A (Glycophorin-A, GPA, GYPA, CD235a, CD235a Antigen, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS, MN Sialoglycoprotein, PAS-2, Sialoglycoprotein alpha)

Anti -Glycophorin A (Glycophorin-A, GPA, GYPA, CD235a, CD235a Antigen, GPErik, GpMiIII,
GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS, MN Sialoglycoprotein, PAS-2,
Sialoglycoprotein alpha)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. The antibody agglutinates untreated RBCs but it failes to agglutinate papain-treated cells. The antibody HIR2 significantly binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
Catalog #G8177-14
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.1mg labels 10e6 human peripheral blood leukocytes.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone NoHIR2
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 15mM sodium azide, pH7.4.
PurityPurified by Protein G affinity chromatography.
ImmunogenCD235a/Glycophorin A.
SpecificityRecognizes N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesGlycophorin A (MN Blood Group),


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