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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified FC

The sialoglycoprotein designated “A” is more commonly known as Glycophorin A and is found on the membrane of cells of the erythroid lineage1.
Catalog #	G8177-25
Applications	Can be used for identifying and gating erythrocytes, erythroblasts and reticulocytes2 or as a marker for identifying leukemias of erythroid origin (AML-M6)3,4.
Recomended Dilution	Flow cytometry: use 10ul direct from the vial per 1x10e7 erythrocytes or per 5x10e5 nucleated cells.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	2Q1511
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. PE conjugated.
Purity	Purified from cell culture supernatant. Tested by electrophoresis and flow cytometry.
Immunogen	Human red blood cells followed by purified glycophorin A.
Specificity	Binds to glutaraldehyde fixed or neuraminidase treated erythrocytes but not to papain, trypsin or pronase treated erythrocytes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.