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You are here:Home » Antibodies » Abs to CD Markers » Anti -Glycophorin A (PE)

Anti -Glycophorin A (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified FC
The sialoglycoprotein designated A is more commonly known as Glycophorin A and is found on the membrane of cells of the erythroid lineage1.
Catalog #G8177-25
ApplicationsCan be used for identifying and gating erythrocytes, erythroblasts and reticulocytes2 or as a marker for identifying leukemias of erythroid origin (AML-M6)3,4.
Recomended DilutionFlow cytometry: use 10ul direct from the vial per 1x10e7 erythrocytes or per 5x10e5 nucleated cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No2Q1511
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. PE conjugated.
PurityPurified from cell culture supernatant. Tested by electrophoresis and flow cytometry.
ImmunogenHuman red blood cells followed by purified glycophorin A.
SpecificityBinds to glutaraldehyde fixed or neuraminidase treated erythrocytes but not to papain, trypsin or pronase treated erythrocytes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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