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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

Glycoprotein (gp) 340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp340 has been shown to bind SP-D in a calcium-dependent manner through a protein-protein interaction (1). Like SP-D, gp340 is found in the lungs and immunohistochemistry analysis shows high expression in alveolar macrophages (1). An identical protein, salivary agglutinin, is present in saliva (2-4) and both gp340 and salivary agglutinin are encoded by the gene DMBT1 (5-7).
Catalog #	G8179-10
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: A strong reaction is seen when gp340 is directly coated onto the microtiter well.
	Western Blot: 1:200-1:10,000 (1-4, 6). Strongly reacts with gp340 in both reduced and non-reduced forms in Western Blot after SDS-PAGE.
	Immunohistochemistry: see references 1,5,6
	Optimal dilutions to be determined by the researcher.
Culture Medium	RMPI 1640 with 10% fetal calf serum.
Hybridoma	X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F121
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4,15mM sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Native Glycoprotein 340 (gp340) purified from the bronchoalveolar lavage fluid from patients with pulmonary alveolar
proteinosis, adsorbed onto aluminum hydroxide gel.
Specificity	Recognizes human Glycoprotein 340. Reacts with a carbohydrate (sialic acid) epitope. Crossreacts with salivary MUC7 in Immunohistochemistry (4).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.