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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

Glycoprotein 340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp340 has been shown to bind SP-D in a calcium dependent manner through a protein-protein interaction. Like SP-D, gp340 is mainly found in lung tissue and immunohistochemistry revealed high expression in alveolar macrophages (3). An identical protein is present in saliva (salivary agglutinin) and both forms are encoded by the gene DMBT1 (6).
Catalog #	G8179-11
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:8000
	Western Blot: 1:500
	Immunohistochemistry: 1:50
	Optimal dilutions to be determined by the researcher.
Culture Medium	RPMI 1640 with 10% fetal calf serum
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F122
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A/G affinity chromatography from culture supernatant.
Immunogen	Native gp340 purified from bronchioaveolar lavage from proteinosis patients.
Specificity	Recognizes human Glycoprotein 340 (gp340). In Western Blot after SDS-PAGE, reacts strongly with gp340 in non-reduced forms.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.