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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Glycoprotein 340 (gp340)

Anti -Glycoprotein 340 (gp340)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Glycoprotein 340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp340 has been shown to bind SP-D in a calcium dependent manner through a protein-protein interaction. Like SP-D, gp340 is mainly found in lung tissue and immunohistochemistry revealed high expression in alveolar macrophages (3). An identical protein is present in saliva (salivary agglutinin) and both forms are encoded by the gene DMBT1 (6).
Catalog #G8179-11
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:8000
Western Blot: 1:500
Immunohistochemistry: 1:50
Optimal dilutions to be determined by the researcher.
Culture MediumRPMI 1640 with 10% fetal calf serum
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F122
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenNative gp340 purified from bronchioaveolar lavage from proteinosis patients.
SpecificityRecognizes human Glycoprotein 340 (gp340). In Western Blot after SDS-PAGE, reacts strongly with gp340 in non-reduced forms.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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