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You are here:Home » Antibodies » Abs to Proteoglycans » Anti -Glypican 4, aa367-382 (K-glypican, UNQ474/PRO937, GPC4)

Anti -Glypican 4, aa367-382 (K-glypican, UNQ474/PRO937, GPC4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH
Glypican-4, previously known as K-glypican, is a GPI-linked cell surface heparan sulfate proteoglycan of the glypican gene family, that comprises 6 members at present. Its function is only partially understood at present. However, glypican-4 seems to be involved in the control of cell growth and diffe-rentiation, especially in the development of the central nervous system and the kidney. Furthermore, glypican-4 is expressed by haematopoetic stem cells and bone marrow stromal cells. Although the antiserum was prepared against a peptide from hu man glypican-4, due to the highly conserved structure and se-quence of glypican-4, antiserum anti-peptide 1 (aa88-101) was used successfully to study brain development in the mouse.
Catalog #G8235-52D
ApplicationsSuitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blotting: 1:1000-1:2000
Immunohistochemistry: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.
PuritySerum
ImmunogenSynthetic glypican-4 peptide (aa367-382, -RPHHPEERPTT-AAGTSL-) coupled to KLH (keyhole limpet haemocyanin)
SpecificitySpecific for a core protein epitope (aa 367-382) of human glypican- 4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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