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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B IP

The Golgi apparatus functions in the modification, organization and transport of proteins and membranes targeted to other parts of the cell, such as the plasma membrane, lysosomes and endosomes. This regulated transport is important for appropriate protein localization, secretion and signal transduction. Members of the Golgin family of proteins, including GM130, Giantin, p115 and GRASP65, are defined by their presence in the Golgi matrix and by their long coiled coil domains. Golgin function, which is regulated in part by small GTPases of the Rab and Arl families, includes establishing and maintaining Golgi structure as well as transport (reviewed in 1). The Golgi cisternae are stacked and linked laterally to form a ribbon. GRASP65 and GM130 are required for membrane fusion events that mediate ribbon formation during Golgi assembly. These lateral fusion events allow for uniform distribution of Golgi enzymes (2). GM130 and Giantin interact with the transport factor p115 to facilitate endoplasmic reticulum (ER)-Golgi transport (3). GM130 is also involved in the transport of the Ether-a-go-go-Related (hERG) potassium ion channel, whose inappropriate localization may be an underlying cause in long Q-T syndrome, a hereditary and potentially fatal cardiac arrhythmia (4). Further, GM130 was implicated in signal transduction regulating invasion, migration and cell polarization via its interaction with and activation of serine/threonine kinases YSK1 and MST4 (5).
Catalog #	G8238-01
Applications	Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western blot 1:1000
	Immunoprecipitation 1:25
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
Purity	Serum
Immunogen	Synthetic peptide (KLH-coupled) corresponding to the amino-terminal sequence of human GM130.
Specificity	Detects endogenous levels of total GM130 protein. Species Crossreactivity: Human and monkey.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.