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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation (such as cyclic AMP, calcium and diacylglycerol), protein phosphorylation, ion channel activation, gene induction, cell growth and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of alpha subunits (ai1, ai2, and ai3).
Catalog #	G8599-01
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Positive Control	IMR-5 cells; brain
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	0.N.305
Host	Mouse
Source	Rat
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, before addition of glycerol to 40%. Also available without BSA and azide. See G8599-01X.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Partially purified Gai1 protein from rat brain (39-42kD). Cellular Localization: Cell membrane.
Specificity	Recognizes Rat Gai1. Does not crossreact with Transducin, Gao, Gai2, Gai3, or Gas. ADP-ribosylation of ai1 proteins does not alter the reactivity. Species Crosseactivity: mouse, bovine, guinea pig and human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.