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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Go belongs to the pertussis toxin sensitive G-proteins. It consists of two subtypes of a-subunits (ao1 and a2) derived from alternative splicing of a single gene. ao1 differs from ao2 within the C-terminal third of the deduced amino acid sequence. Some of the literature suggests that Transducin is the generic name for the heterotrimeric G protein or possibly Gt. Transducin is composed of several subunits. G proteins are further categorized into different classes, including Go and Gi, based on their aa sequence. Goa is a subunit of Go. Goa could be one subgroup of Transducin. However, there is some literature stating that Go, Gi, and Transducin are all separate groups of G proteins.
Catalog #	G8599-03
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions	Western Blot 1:1000. Detects a single protein band of 39-42kD in bovine and rat membranes.
	Optimal dilutions to be determined by researcher.
Source	Ascites
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2Q253
Host	Mouse
Source	Bovine
Concentration	Not determined
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Ascites
Immunogen	Bovine brain Goa purified by the method of Sternweis and Robishaw
Specificity	Recognizes Goa. Species Crossreactivity: Strong crossreactivity with bovine (100%) and human (58%). Slight crossreactivity with guinea pig (24%) and rat (26%). Does not crossreact with mouse. Specificity confirmed by Western Blot versus bovine Gat and Gsa; rat Goa, Gia-1, Gia-2 and Gia-3; mouse GoaA and GoaB
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.