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You are here:Home » Antibodies » Abs to G Proteins » Anti -G-Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 / guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2)

Anti -G-Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein), alpha
inhibiting activity polypeptide 1 / guanine nucleotide binding protein (G protein), alpha
inhibiting activity polypeptide 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B IH
GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of alpha-subunits (alphai1, alphai2 and alphai3).
Catalog #G8599-05A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:100
Optimal dilutions to be determined by the researcher.
Positive ControlL6 Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.08% sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to aa345-354 of Gi alpha1 and 346-355 of Gi alpha2.
SpecificityRecognizes human G protein subunit alphai1 and alpha2. Species Crossreactivity: mouse, rat, bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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