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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP

Gai2 is a 40--41kD alpha-subunit of heterotrimeric protein, that shares sequence homology with Gai3 and Gai1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence, interfering with receptor coupling. Gi2 is expressed widely in tissues and cell lines. Activation of PLC-B by Gai-coupled receptors is the result of release of GBy subunits from Gai. Gai2 regulates potassium channel activation. Mutation at Arg 179 has been identified in tumors. This and the Q205L mutant inhibit adenyl cyclase.
Catalog #	G8599-07A
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	E. coli-expressed human Gai2 (aa 231-351)
Specificity	Reacts human Gai2 protein. Crossreacts with Gai3, and partially with Gai1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.