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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation (such as cyclic AMP, calcium and diacylglycerol), protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation.
Catalog #	G8599-08
Applications	Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilutions	Western Blot: 1:500–1:1000, with detection by 125I-labeled anti-IgG.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	2Q256
Host	Mouse
Source	Bovine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Purity	Purified
Immunogen	Recombinant Gia-2
Specificity	Recognizes bovine Gia-2 at ~36-49kD. Specificity confirmed by Western Blot versus bovine Gat, rat Goa, rat Gia-1, Gia-2, rat Gia-3, bovine Gsa and mouse GoaA and GoaB. Species sequence homology: mouse, 69%; rat, 69%; human, 62%, guinea pig, 43%
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.