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You are here:Home » Antibodies » Abs to G Proteins » Anti -G Protein, i alpha 2, (Gia2)

Anti -G Protein, i alpha 2, (Gia2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #G8599-09
ApplicationsSuitable for use in ELISA and Immunoblot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1-2ug/ml. Detects Gia2 from a rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G8599-09 (1.5ug/ml). Proteins were visualized using a goat anti-mouse IgG labeled with HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Immunoblot Protocol1. Perform SDS-PAGE on a microsomal membrane preparation. Transfer the proteins to nitrocellulose. Wash the blotted nitrocellulose twice with water.
2. Block the blotted nitrocellulose in freshly prepared PBS, 3% nonfat dry milk (PBS-MLK) for 60 minutes at RT with constant agitation.
3. Incubate the nitrocellulose with 1-2ug/ml of G8599-09, diluted in freshly prepared PBS-MLK, overnight at 4°C with constant agitation.
4. Wash the nitrocellulose twice with water.
5. Incubate the nitrocellulose in the secondary reagent of choice (a goat anti-mouse IgG labeled with HRP was used) in PBS-MLK for 1.5 hours at RT with constant agitation.
6. Wash the nitrocellulose with water twice.
7. Wash the nitrocellulose in PBS, 0.05% Tween-20 for 10 minutes.
8. Rinse the nitrocellulose in 4-5 changes of water for 2 hours.
9. Use detection method of choice. Enhanced chemiluminescence was used.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4i41
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.13M Tris, pH 7.4, 0.32M acetic acid, 30% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenFull length, rat Gia2 puri ed by ion exchange chromatography.
SpecificityRecognizes rat Gia2, Mr 41kD. Broad species crossreactivity expected due to the highly conserved nature of Gia2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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