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You are here:Home » Antibodies » Abs to G Protein Receptors » Anti -G-Protein Coupled Receptor 49 (GPCR49, GPR49, Leucine-rich Repeat Containing G-protein Coupled Receptor 5, Lgr5)

Anti -G-Protein Coupled Receptor 49 (GPCR49, GPR49, Leucine-rich Repeat Containing
G-protein Coupled Receptor 5, Lgr5)


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Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC
G-protein coupled receptor GPR49 (also known as Leucine-rich repeat containing G-protein coupled receptor 5, Lgr5) is closely related to members of the glycoprotein hormone receptor subfamily. It is expressed in skeletal muscle, placenta, spinal cord, and various regions of the brain. GPR49 is reported to possibly mark stem cells in multiple adult tissues and is over expressed in cancers. G protein-coupled receptor GPR49 is an Orphan-A GPCR with an unknown ligand. GPR49 has been reported to be expressed in brain, skeletal muscle, placenta and spinal cord. ESTs have been isolated from normal human brain (amygdala), embryo, and placenta libraries, and from human germ cell, uterus and brain (neuroblastoma) cancer libraries.
Catalog #G8600-53B
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Unsuitable for use in Immunofluorescence/ Immunocytochemistry Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunoprecipitation: 1:20
Immunohistochemistry (Paraffin): 1:100-1:250
Flow Cytometry: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlsFetal skeletal muscle lysate, human muscle tissue, human ovarian, carcinoma tissue
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No9j103
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
ImmunogenSynthetic peptide corresponding to residues in human GPR49
SpecificityRecognizes human GPR49 at ~100kD. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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