Anti -G Protein Coupled Receptor Kinase-Interactor 2 (GRK-interacting Protein 2, GIT2, ARF
GTPase-activating Protein GIT2, ARF GAP GIT2, Cool-interacting Tyrosine-phosphorylated
Protein 2, CAT2, CAT-2, DKFZp686G01261, KIAA0148, MGC760) (Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH IF |
|
| This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. | | | Catalog # | G8602-72B | | Applications | Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (paraffin): 1.5ug/ml | | ELISA: 1ug/ml-3ng/ml | | Western Blot: 1:500-1:1000 | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Sp2/0 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 10C415 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant protein corresponding to a portion of amino acids within aa1-472 of human GIT2. | | Specificity | Recognizes human GIT2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | G Protein-coupled Receptor Kinase Interacting ArfGAP 2 |
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