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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Gr-1 (GR1, Myeloid Differentiation Antigen, Lymphocyte Antigen 6 Complex Locus G, Lymphocyte Antigen 6G, Ly6g, Ly-6G, Ly-6G.1)

Anti -Gr-1 (GR1, Myeloid Differentiation Antigen, Lymphocyte Antigen 6 Complex Locus G,
Lymphocyte Antigen 6G, Ly6g, Ly-6G, Ly-6G.1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IP IH FC
The Gr-1 antigen is a 21-25kD cell surface protein that belongs to the Ly-6 family of proteins. The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Expression of Gr-1 on a subpopulation of lymphocytes has also been reported.
Catalog #G8750-01A2
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Westen Blot. Reported to deplete mouse mature neutrophils in vivo. Catalog #G8750-01A1 is recommended for functional studies. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul.
Immunohistochemistry: Frozen sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone NoRB6-8C5
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenNormal murine bone marrow cells.
SpecificityRecognizes mouse Gr-1 antigen. Reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been demonstrated.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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