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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Gr-1 (GR1, Myeloid Differentiation Antigen, Lymphocyte Antigen 6 Complex Locus G, Lymphocyte Antigen 6G, Ly6g, Ly-6G, Ly-6G.1) (Biotin)

Anti -Gr-1 (GR1, Myeloid Differentiation Antigen, Lymphocyte Antigen 6 Complex Locus G,
Lymphocyte Antigen 6G, Ly6g, Ly-6G, Ly-6G.1) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Expression of Gr-1 on a subpopulation of lymphocytes has also been reported.
Catalog #G8750-01A5
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone NoRB6-8C5
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenNormal mouse bone marrow cells.
SpecificityRecognizes mouse Gr-1 antigen. Reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been demonstrated.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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