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You are here:Home » Growth Factors, Cytokines » Growth Factors-GM-CSF » Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl

Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst
Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony
Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl

Pricing

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Specifications

Recombinant Human GM-CSF produced in insect cells is a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton.
Catalog #G8950-10B
Recombinant Human GM-CSF is fused to a C-terminal His -tag (6x His) and purified by proprietary chromatographic techniques.
Amino acid sequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been completely removed enzymatically.
Biological ActivityRecombinant GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is 0.1ng/ml, corresponding to a Specific Activity of 1x10e7 IU/mg.
Protein contentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O or buffer of choice and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight14.48kD
SourceRecombinant, Human, Sf9 insect cells
Purity98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
FormSupplied as a lyophilized powder without additives. It is recommended to reconstitute the lyophilized GM-CSF in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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