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You are here:Home » Molecular Biology » Growth Factors-G-CSF » Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)

Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)

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Specifications

G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.
Catalog #G8950-15
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is 0.1ng/ml, corresponding to a specific activity of 1x10e7 units/mg.
AA SequenceTPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O and a carrier protein (0.1% BSA/HSA). Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
Molecular Weight18.7kD
SourceHuman, recombinant from E. coli
Purity98% by SDS-PAGE. Endotoxin: 0.1 ng/ug
Concentration~1mg/ml
FormSupplied as a lyophilized powder from 5mM sodium citrate, pH 4.0. Reconstitute in ddH2O and a carrier protein to 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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