Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Growth Factors, Cytokines » Growth Factors-G-CSF » Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)

Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

A glycoprotein of MW 20kD containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.
Catalog #G8950-15A
Recombinant Human Granulocyte Colony Stimulating Factor is a glycosylated, polypeptide chain containing 174 amino acids and having a molecular mass of 20kD.
Biological ActivityG-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is less then 0.1 ng/ml, corresponding to a Specific Activity of 1.27 x 10e8 IU/mg.
Protein ContentG-CSF protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Human Granulocyte Colony Stimulating Factor as a Reference Standard.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Thr-Pro-Leu-Gly-Pro.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceRecombinant, Human expressed in CHO cells.
Purity98% by RP-HPLC or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
FormSupplied as a lyophilized powder in 10mM HCl, pH 6.5, Tween 20, mannitol, L-arginine, phenylalanine, methionine.
Reconstitution
Reconstitute in sterile dH2O not less than 0.1mg/ml. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Can be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links