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You are here:Home » Growth Factors, Cytokines » Growth Factors-G-CSF » Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin)

Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF, GCSF, C17orf33, Colony
Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931,
Pluripoietin)

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Specifications

Catalog #G8950-15F
SourceRecombinant corresponding to 175aa of human Granulocyte-Colony Stimulating Factor expressed in E.coli is a single,non-glycosylated, polypeptide chain.
Molecular Weight~18.8kD
Biological ActivityThe ED50, calculated by the dose-dependent proliferation of mouse NFS-60 indicator cells (measured by 3H-thymidine uptake) is less then 0.1ng/ml, corresponding to a Specific Activity of 1x108 IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 0.815 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Recombinant corresponding to G-CSF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~18.8kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
ConcentrationNot determined
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesGranulocyte Colony-stimulating Factor


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