Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF, GCSF, C17orf33, Colony
Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931,
Pluripoietin)
Pricing
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| Catalog # | G8950-15F | | Source | Recombinant corresponding to 175aa of human Granulocyte-Colony Stimulating Factor expressed in E.coli is a single,non-glycosylated, polypeptide chain. | | Molecular Weight | ~18.8kD | | Biological Activity | The ED50, calculated by the dose-dependent proliferation of mouse NFS-60 indicator cells (measured by 3H-thymidine uptake) is less then 0.1ng/ml, corresponding to a Specific Activity of 1x108 IU/mg. | | Dimmers and Aggregates | 1% (SDS-PAGE) | | Protein Content | Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 0.815 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Recombinant corresponding to G-CSF as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~18.8kD | | Purity | ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) | | Concentration | Not determined | | Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Granulocyte Colony-stimulating Factor |
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Download Specifications for Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin) |
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