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Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)
Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)
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| Human granulocyte colony-stimulating factor(hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. | | | | | | Molecular Weight: 18.8kD (175amino acids including N-terminal methionine) | | | | | | Sequence: | | | MTPLGPASSL/ PQSFLLKCLE/ QVRKIQGDGA/ ALQEKLCATY/ KLCHPEELVL/ LGHSLGIPWA/ PLSSCPSQAL/ QLAGCLSQLH/ SGLFLYQGLL/ QALEGISPEL/ GPTLDTLQLD/ VADFATTIWQ/ QMEELGMAPA/ LQPTQGAMPA/ FASAFQRRAG/ GVLVASHLQS/ FLEVSYRVLR/ HLAQP | | | Catalog # | G8950-15X | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 18.8kD | | Source | Human, Recombinant E. coli | | Purity | 95% by SDS PAGE | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4 | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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