| Granulocyte Colony Stimulating Factor (G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Murine G-CSF is a 20kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins , TNF , Interleukin-1 and GM-CSF . Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17. |
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| Catalog # | G8950-16B |
| Recombinant Murine G-CSF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 179 amino acids and having a molecular mass of 19kD. |
| Protein quantitation was carried out by two independent methods | 1. UV spectroscopy at 280nm using the absorbency value of 0.51 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| 2. Analysis by RP-HPLC, using a calibrated solution of G-CSF as a Reference Standard. |
| Dimers and aggregates | 1% as determined by silver-stained SDS-PAGE gel. |
| Biological Activity | G-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is 0.05ng/ml, corresponding to a Specific Activity of 2x10e7 IU/mg. |
| AA Sequence | MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer |
| Molecular Weight | 19.0kD |
| Source | Recombinant, Murine (E. coli) |
| Purity | 95% by RP-HPLC, Anion-exchange FPLC, reducing and non-reducing SDS-PAGE silver stained gel. Endotoxin: 0.1ng/ug (1EU/ug) |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder. No stabilizing proteins added. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of 100ug/ml, which can then be further diluted to other aqueous solutions. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
